confocal immunofluorescence microscopy (Carl Zeiss)
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Confocal Immunofluorescence Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Staining:Article Title: Identification of ORF1B as a unique nonstructural protein for fowl adenovirus serotype 4. Article Snippet: Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), is a double-stranded DNA virus.. Although many structural proteins have been deeply studied, the coding potential of some other open reading frames (ORFs) and the biological functions of their products during virus infection have not been fully elucidated.. Here, a unique nonstructural protein ORF1B of FAdV-4 was identified and its expression kinetics along infection was analyzed. Article Title: 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8. Article Snippet: Senecavirus A (SVA), an important emerging porcine virus, has outbreaks in different regions and countries each year, becoming a virus with global prevalence.. SVA infection has been reported to induce macroautophagy/autophagy; however, the molecular mechanisms of autophagy induction and the effect of SVA on autophagy remain unknown.. This study showed that SVA infection induced the autophagy process in the early stage of SVA infection, and the rapamycin-induced autophagy inhibited SVA replication by degrading virus 3 C protein. Article Title: Association of polymorphisms in the vitamin D receptor gene with susceptibility to and severity of hand, foot, and mouth disease caused by coxsackievirus A16. Article Snippet: 1 Department of Hepatobiliary Surgery, ZhongShan Hospital of Xiamen University, Fujian, China 2 Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, ZhongShan Hospital of Xiamen University, Fujian, China 3 Graduate College of Fujian Medical University, Fuzhou, Fujian, China 4 Department of Pathology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China 5 Medicine Clinical Laboratory, Xiamen Xianyue Hospital, Fujian, China Fluorescence:Article Title: Identification of ORF1B as a unique nonstructural protein for fowl adenovirus serotype 4. Article Snippet: Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), is a double-stranded DNA virus.. Although many structural proteins have been deeply studied, the coding potential of some other open reading frames (ORFs) and the biological functions of their products during virus infection have not been fully elucidated.. Here, a unique nonstructural protein ORF1B of FAdV-4 was identified and its expression kinetics along infection was analyzed. Article Title: 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8. Article Snippet: Senecavirus A (SVA), an important emerging porcine virus, has outbreaks in different regions and countries each year, becoming a virus with global prevalence.. SVA infection has been reported to induce macroautophagy/autophagy; however, the molecular mechanisms of autophagy induction and the effect of SVA on autophagy remain unknown.. This study showed that SVA infection induced the autophagy process in the early stage of SVA infection, and the rapamycin-induced autophagy inhibited SVA replication by degrading virus 3 C protein. Immunofluorescence:Article Title: Identification of ORF1B as a unique nonstructural protein for fowl adenovirus serotype 4. Article Snippet: Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), is a double-stranded DNA virus.. Although many structural proteins have been deeply studied, the coding potential of some other open reading frames (ORFs) and the biological functions of their products during virus infection have not been fully elucidated.. Here, a unique nonstructural protein ORF1B of FAdV-4 was identified and its expression kinetics along infection was analyzed. Article Title: b-resorcylic acid released by Limosilactobacillus reuteri protects against cisplatin-induced ovarian toxicity and infertility Article Snippet: .. Article Title: 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8. Article Snippet: Senecavirus A (SVA), an important emerging porcine virus, has outbreaks in different regions and countries each year, becoming a virus with global prevalence.. SVA infection has been reported to induce macroautophagy/autophagy; however, the molecular mechanisms of autophagy induction and the effect of SVA on autophagy remain unknown.. This study showed that SVA infection induced the autophagy process in the early stage of SVA infection, and the rapamycin-induced autophagy inhibited SVA replication by degrading virus 3 C protein. Article Title: POLM inhibits porcine epidemic diarrhea virus replication by degrading multiple viral structural proteins. Article Snippet: After immobilizing in 4% paraformaldehyde (P6148), the cell samples were permeabi lized with 0.1% Triton X-100 (9284, both Sigma-Aldrich), followed by 60 min of incuba tion with 3% bovine serum albumin-diluted primary and secondary antibodies at 37°C. .. Finally, cell observation was performed by Article Title: Association of polymorphisms in the vitamin D receptor gene with susceptibility to and severity of hand, foot, and mouth disease caused by coxsackievirus A16. Article Snippet: 1 Department of Hepatobiliary Surgery, ZhongShan Hospital of Xiamen University, Fujian, China 2 Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, ZhongShan Hospital of Xiamen University, Fujian, China 3 Graduate College of Fujian Medical University, Fuzhou, Fujian, China 4 Department of Pathology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China 5 Medicine Clinical Laboratory, Xiamen Xianyue Hospital, Fujian, China Article Title: β-resorcylic acid released by Limosilactobacillus reuteri protects against cisplatin-induced ovarian toxicity and infertility. Article Snippet: .. Article Title: EGR1 Suppresses Porcine Epidemic Diarrhea Virus Replication by Regulating IRAV To Degrade Viral Nucleocapsid Protein. Article Snippet: After three washes with PBS, nuclei were stained with DAPI (catalog no. C1002; Beyotime Biotechnology) for 5min at room temperature. .. The cells were observed by Article Title: RILP inhibits proliferation, migration, and invasion of PC3 prostate cancer cells. Article Snippet: In brief, cells grown on coverglasses were washed with PBSCM (PBS containing 1.0 mM CaCl2 and 1.0 mM MgCl2) and then fixed with 3% paraformaldehyde(PFA) at 4 ◦C for 30 min. After sequential washing with PBSCM supplemented with 50 mM NH4Cl, cells were permeabilized with 0.1% saponin (Sigma) in PBSCM for 15–20 min at room temperature, then subjected for immuno-staining using the indicated primary and secondary antibodies. .. Cells expressing GFP-tagged proteins and immuno-labeled proteins were observed and analyzed by Microscopy:Article Title: Identification of ORF1B as a unique nonstructural protein for fowl adenovirus serotype 4. Article Snippet: Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), is a double-stranded DNA virus.. Although many structural proteins have been deeply studied, the coding potential of some other open reading frames (ORFs) and the biological functions of their products during virus infection have not been fully elucidated.. Here, a unique nonstructural protein ORF1B of FAdV-4 was identified and its expression kinetics along infection was analyzed. Article Title: b-resorcylic acid released by Limosilactobacillus reuteri protects against cisplatin-induced ovarian toxicity and infertility Article Snippet: .. Article Title: 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8. Article Snippet: Senecavirus A (SVA), an important emerging porcine virus, has outbreaks in different regions and countries each year, becoming a virus with global prevalence.. SVA infection has been reported to induce macroautophagy/autophagy; however, the molecular mechanisms of autophagy induction and the effect of SVA on autophagy remain unknown.. This study showed that SVA infection induced the autophagy process in the early stage of SVA infection, and the rapamycin-induced autophagy inhibited SVA replication by degrading virus 3 C protein. Article Title: POLM inhibits porcine epidemic diarrhea virus replication by degrading multiple viral structural proteins. Article Snippet: After immobilizing in 4% paraformaldehyde (P6148), the cell samples were permeabi lized with 0.1% Triton X-100 (9284, both Sigma-Aldrich), followed by 60 min of incuba tion with 3% bovine serum albumin-diluted primary and secondary antibodies at 37°C. .. Finally, cell observation was performed by Article Title: Association of polymorphisms in the vitamin D receptor gene with susceptibility to and severity of hand, foot, and mouth disease caused by coxsackievirus A16. Article Snippet: 1 Department of Hepatobiliary Surgery, ZhongShan Hospital of Xiamen University, Fujian, China 2 Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, ZhongShan Hospital of Xiamen University, Fujian, China 3 Graduate College of Fujian Medical University, Fuzhou, Fujian, China 4 Department of Pathology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China 5 Medicine Clinical Laboratory, Xiamen Xianyue Hospital, Fujian, China Article Title: β-resorcylic acid released by Limosilactobacillus reuteri protects against cisplatin-induced ovarian toxicity and infertility. Article Snippet: .. Article Title: EGR1 Suppresses Porcine Epidemic Diarrhea Virus Replication by Regulating IRAV To Degrade Viral Nucleocapsid Protein. Article Snippet: After three washes with PBS, nuclei were stained with DAPI (catalog no. C1002; Beyotime Biotechnology) for 5min at room temperature. .. The cells were observed by Article Title: RILP inhibits proliferation, migration, and invasion of PC3 prostate cancer cells. Article Snippet: In brief, cells grown on coverglasses were washed with PBSCM (PBS containing 1.0 mM CaCl2 and 1.0 mM MgCl2) and then fixed with 3% paraformaldehyde(PFA) at 4 ◦C for 30 min. After sequential washing with PBSCM supplemented with 50 mM NH4Cl, cells were permeabilized with 0.1% saponin (Sigma) in PBSCM for 15–20 min at room temperature, then subjected for immuno-staining using the indicated primary and secondary antibodies. .. Cells expressing GFP-tagged proteins and immuno-labeled proteins were observed and analyzed by Expressing:Article Title: RILP inhibits proliferation, migration, and invasion of PC3 prostate cancer cells. Article Snippet: In brief, cells grown on coverglasses were washed with PBSCM (PBS containing 1.0 mM CaCl2 and 1.0 mM MgCl2) and then fixed with 3% paraformaldehyde(PFA) at 4 ◦C for 30 min. After sequential washing with PBSCM supplemented with 50 mM NH4Cl, cells were permeabilized with 0.1% saponin (Sigma) in PBSCM for 15–20 min at room temperature, then subjected for immuno-staining using the indicated primary and secondary antibodies. .. Cells expressing GFP-tagged proteins and immuno-labeled proteins were observed and analyzed by |

